Mathematical modelling of the automated FADU assay for the quantification of DNA strand breaks and their repair in human peripheral mononuclear blood cells.

DNA strand breaks are one of the most common genotoxic lesions and they can also arise as intermediates of DNA repair activity. We have previously published an automated method for measuring DNA strand breaks based on fluorimetric detection of alkaline DNA unwinding [1], and here we present a mathematical model of the FADU assay, which enables to an analytic expression for the relation between measured fluorescence and the number of strand breaks.

Junk M, Salzwedel J, Sindlinger T, Bürkle A, Moreno-Villanueva M.

BMC Biophys. 2014 Sep 9;7:9. doi: 10.1186/s13628-014-0009-z. eCollection 2014. PMID: 26085926