Quality control data of physiological and immunological biomarkers measured in serum and plasma.

In two work packages of the MARK-AGE project, 37 immunological and physiological biomarkers were measured in 3637 serum, plasma or blood samples in five batches during a period of 4 years. The quality of the serum and plasma samples was very good as judged by the low number of biomarker measurements (only 0.2%) that were rejected because of a high hemolysis, icteria or lipemia of the samples. Using quality control samples, day-to-day and batch variations were determined. The mean inter-assay variation of the five batches were all below 8%, with an average inter-assay coefficient of variation of all biomarkers of 4.0%.

Jansen E, Beekhof P, Cremers J, Weinberger B, Fiegl S, Toussaint O, Bernhard J, Gonos E, Capri M, Franceschi C, Sikora E, Moreno-Villanueva M, Breusing N, Grune T, Bürkle A, Dollé ME.

Mech Ageing Dev. 2015 Nov;151:54-9. doi: 10.1016/j.mad.2015.06.004. Epub 2015 Jul 9. PMID: 26166476