High-resolution quantitative metabolome analysis of urine by automated flow injection NMR.

Metabolism is essential to understand human health. To characterize human metabolism, a high-resolution read-out of the metabolic status under various physiological conditions, either in health or disease, is needed. Metabolomics offers an unprecedented approach for generating system-specific biochemical definitions of a human phenotype through the capture of a variety of metabolites in a single measurement. The emergence of large cohorts in clinical studies increases the demand of technologies able to analyze a large number of measurements, in an automated fashion, in the most robust way. NMR is an established metabolomics tool for obtaining metabolic phenotypes.

Da Silva L, Godejohann M, Martin FP, Collino S, Bürkle A, Moreno-Villanueva M, Bernhardt J, Toussaint O, Grubeck-Loebenstein B, Gonos ES, Sikora E, Grune T, Breusing N, Franceschi C, Hervonen A, Spraul M, Moco S.

Anal Chem. 2013 Jun 18;85(12):5801-9. doi: 10.1021/ac4004776 Epub 2013 May 29. PMID: 23718684