An exploratory study on the effect of choline and folate deficiency on levels of vascularization proteins and transcription factors in first trimester trophoblast HTR-8/SVneo cells

We studied the effect of choline and folate deficiencies on levels of predetermined placental proteins during early development. HTR-8/SVneo cells were incubated under choline and folate deficiency conditions and levels of some placental proteins were measured using ELISA methods. As a conlcusion, LRP2 and CDX2 are likely to be molecular targets for early choline and folate deficiencies in human trophoblast cells. The results should be confirmed in animal models and in other models of placental cells. 

Engelhart K, Pfitzner I, Obeid R

J Obstet Gynaecol Res. 2023 Jan 15.doi: 10.1111/jog.15555.