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Oxidative and inflammatory processes: key drivers of aging and disease

Oxidative and inflammatory processes underlie many ageing and disease processes. However, many plants contain secondary metabolites with anti-oxidative and anti-inflammatory potential contributing to the reduction of these processes. We have established various tests to support claims for food supplements and cosmetics.

 

Rapid and reliable determination of antioxidant capacity

The FluoroScan method provides qualitative insights into the antioxidant capacity of the test substances. Performed on a multi-well scale, it is particularly suitable for screening potential new active ingredients or combinations of aforementioned. For testing lipophilic substances, BioTeSys employs specialized solubilization techniques.

To determine the antioxidant capacity, BioTeSys uses a (non-)fluorescent dye that is oxidised intracellularly by free radicals and thus stimulated to fluorescence. The intensity of fluorescence is a measure of the antioxidant capacity.

Advantages

  • Sensitive method for detecting intracellularly formed radicals
  • Rapid screening of large test groups
  • Determination of the antioxidant capacity of isolated test substances individually or in combination
  • Assessment of the antioxidant capacity of complex natural product extracts

The FluoroScan method quickly and reliably provides initial assessments of the antioxidant potential of a test substance. For more in-depth testing, analyses of GSH reductase activity or the redox status can be supplemented using the GSH:GSSG ratio.

Anti-inflammatory capacity

Inflammation is a self-regulated immune process mediated by cytokines and occurs in response to various infections and stimuli. It also plays a crucial part in wound and tissue repair. An indication of a test substance’s anti-inflammatory potential can therefore be the inhibition of the expression of key inflammatory cytokines, such as IL-6, IL-8 and TNFα. These assays are valuable tools in assessing the potential of substances to modulate inflammatory processes effectively.