Antioxidant capacity

Antioxidant and anti-inflammatory capacity

 

Oxidative and inflammatory processes underlie many aging and disease processes. However, many plants contain secondary ingredients with antioxidant and anti-inflammatory potential, contributing to the reduction of these processes. We have established various tests to support claims for food supplements and cosmetics.

Rapid and reliable determination of antioxidant capacity

The FluoroScan method provides qualitative information on the antioxidant capacity of test substances. Performed on a multi-well scale, it is particularly suitable for screening potential new active ingredients or mixtures of active ingredients. Biotesys uses several specialized solubilization methods to test lipophilic test substances. 

To determine the antioxidant capacity, Biotesys uses a (non-)fluorescent dye that is oxidized intracellularly by free radicals and thus stimulated to fluoresce. The fluorescence is a measure of the antioxidant capacity of the cells.


Advantages

• Sensitive method for detecting intracellularly formed radicals
• Fast screening of large test groups
• Determination of the antioxidant capacity of isolated test substances, individually or in combination
• Determination of the antioxidant capacity of complex natural product extracts

The FluoroScan method provides fast and reliable initial assessments of the antioxidant potential of a test substance. For more in-depth testing, analyses of GSH reductase activity or the redox status can be supplemented using the GSH:GSSG ratio.


Anti-inflammatory capacity

Inflammation is an immune process that is self-regulated by cytokines, among other things, and occurs in response to various infections and other stimuli, or as an important component of wound and tissue repair. An indication of the anti-inflammatory potential of a test substance can therefore be the inhibition of the expression of key inflammatory cytokines, such as IL-6, IL-8, and TNFα.